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Briefly, confluent cells were serum starved overnight, followed by administration of 100 nM insulin for 5 hours

Risk-stratified prescribing should therefore align agent selection, dose escalation, and monitoring intensity with patient-specific vulnerabilities, particularly gastrointestinal tolerability, risk of renal dehydration, gallbladder history, retinopathy risk during rapid glycaemic improvement, and frailty or sarcopenia risk

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doi: 10.1210/er.2011-1052 14 DuringMJCaoLZuzgaDSFrancisSJFitzsimonsHLJiaoXet al

Hydration requirements increase during treatment