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doi:10.1073/pnas.75.3.1260 44
The final solution was vortexed for 5 s and 300 l was plated per well (two wells plated per condition) and the fluorescence was recorded on the BioTek H1 Synergy microplate as described above
Berberine should not be described as a GLP-1 'activator' in the medicinal sense, as any effects on GLP-1 pathways appear to be indirect and considerably less potent than pharmaceutical GLP-1 therapies
It can increase the expression and function of tight junction proteins, such as occludin, claudins, and zonula occludens-1 (ZO-1), which are vital components of the gut barrier (105)
Additional benefits of GLP-1 include the reduction of risk for adverse cardiovascular events in individuals with obesity and or Type 2 Diabetes due to its anti-inflammatory action
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