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Add to this the fact that some individuals are more prone to vitamin B12 deficiency, and the confusion is compounded

Stock solutions are typically prepared in sterile water and diluted into culture medium

In Malaysia, invasive procedures like IV drips should only be performed in licensed medical environments to ensure sterile conditions and correct dosing

these are simple, no-cost visual checks that catch a meaningful fraction of handling problems before they compound into unexplained experimental variance downstream

Most studies use fixed doses, though you'll occasionally see weight-based protocols (110 mcg/kg, or roughly 0.54.5 mcg/lb) in the literature