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& Timmerman, L
doi: 10.1007/s10719-009-9239-8 299 AbdulkhalekSAmithSRFranchukSLJayanthPGuoMFinlayTet al
B12 deficiency more common than most people realize, particularly in people over 40 and those with certain dietary patterns can significantly impair energy and metabolic function
The separation was performed on an Easy-Spray PepMap RSLC C18 analytical column (75 m 50 cm, Thermo Scientific) under gradient conditions starting with 2% Buffer B, held for 5 min, followed by a linear increase to 35% Buffer B over 125 min, reaching 98% Buffer B after a further 2 min, and maintained for 10 min, before re-equilibration to 35% Buffer B after 1 min
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