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22,23 Historically, varicocele has been thought to cause infertility by reducing oxygenation and increasing oxidative stress in the testicular environment, but more recent evidence also implicates inflammatory and immune mechanisms
These are typically mild to moderate and improve with gradual dose increases
These sequences were then run through an R script (Supplementary Materials, bash_DADA2 + IDTAXA_workflow_Illumina-PairedEnd_V1V3.R) which trimmed reverse reads to 260 bp, removed forward reads exceeding a maximum expected error of three, and truncated reverse reads exceeding a maximum expected error of six
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