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Determination of GSH Levels HT-1080 cells were cultured in DMEM containing 10% FBS, 1% supplemented non-essential amino acids and 1% Pen-Strep mixture (all from Gibco), and maintained in a humidified atmosphere with 5% CO 2 at 37C
Pituch, A., Walkowiak, J., & Banaszkiewicz, A
Typical GHK-Cu protocols run 4-8 weeks followed by equivalent off periods
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