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Cheng P-P, Xia J-J, Wang H-L, Chen J-B, Wang F-Y, Zhang Y, et al

GST protein has a strong binding affinity for glutathione, immobilized on agarose and when a mixture of proteins is added to this matrix, the protein of interest (tagged to GST) binds to the glutathione ligand and impurities are removed by washing.Finally, the beads are treated with reduced glutathione (free) which competitively detaches the interaction between immobilized glutathione and the GST-tagged protein of interest from the beads which results in a purified protein.

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