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It should be kept away from strong chelators (such as high levels of EDTA and related sequestrants), reducing sugars, thiol-bearing actives, and high concentrations of direct acids (for example ascorbic acid or alpha-hydroxy acids at low pH), all of which can compete for the copper center or disrupt the peptide-metal bond and shift the characteristic blue color toward brown or colorless degradation products
Smith DB, Johnson KS: Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
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But having a licensed provider walk you through technique in person or virtually is a different level of support
Other symptoms associated with Raynauds disease include the following: Numbness in the hands and feet Changes in skin color to white, then blue Pain and swelling in the hands and feet when warmed 6
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