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These are not clinical arguments
10.1016/j.jinorgbio.2013.05.016 100 RizzolloF.AgostinisP
We conclude that anesthesia and sleep share some neuronal structure in their action and mechanism of anesthesia could be elucidated through sleep study
Membranes were blocked in 5% non-fat milk in tris-buffered saline with 0.1% Tween (TBST) overnight at 4C and subsequently incubated with primary antibodies in 5% BSA in 1X TBST overnight at 4C
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