After washing with PBS, cell nuclei were stained with 4,6-diamino-2-phenyl indole (DAPI) for 5 min, and then cells were imaged by fluorescence microscopy
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Concentrations above 30mg/mL increase peptide-peptide interactions that lead to aggregation within 14 days
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These findings tightly link ROS and Nrf2 to ER oxidative protein folding and calcium signaling, establishing a regulatory mechanism for protein oxidative folding-Nrf2-ER calcium axis (Figure 7)