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Loren Pickart in 1973, GHK-Cu was discovered when Pickart observed that albumin from young donors (age 20-25) could stimulate aged liver tissue to synthesize proteins at a youthful rate, and the active factor was traced to this small copper peptide
In methionine semi-starvation, intracellular acidification also occurs, which indicates that as long as intracellular methionine amount is lower than methionine level in the culture supplied with sufficient methionine, bacterial cytoplasm will be acidified
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