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* Correspondence: Xue Yang [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

1- Free GSH=DTNB+Sample+Substrate 2- Total GSH=DTNB+Sample+Substrate+ NADPH+glutathione reductase 3- GSSG=Total GSH-Free GSH Another method to measure GSSG separately, is to remove the free GSH from DTNB reaction process by modification of free GSH with N-ethylmaleimide (NEM) or vinylpyridine prior to conducting assay