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Cell death in cancer cell lines To evaluate the ability of leading candidate proteases to induce cell death via procaspase-1 and gasdermin D cleavage, we stably integrated X(PC) and X(GD), as well as catalytically inactive variants as negative controls, into cancer cell lines under doxycycline-induced expression (Fig

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Following the starvation period, the cells were exposed to either KRH with 5.6 mM glucose (baseline secretion) or the same buffer containing varying concentrations of l-valine