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While primers sequence for IL8, HSP27 and SOD1 were designed using Primer3 ( and Primer-BLAST ( Primer specificity was verified by running PCR for 40 cycles at 95 C for 10 seconds and 60 C for 60 seconds, followed by gel electrophoresis on a 3% agarose gel stained with SYBR safe DNA gel stain
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Contact your healthcare provider if you suspect you have administered the wrong dose, if you experience symptoms that suggest overdose (severe nausea, persistent vomiting, abdominal pain, dizziness), if your vial concentration has changed and you are unsure about the new calculations, or if you have been prescribed a dose that seems unusually high or low compared to standard protocols
If needed (prior to cap removal), the pen can be kept from 8C to 30C (46F to 86F) for up to 28 days
thus, they may be more accurately interpreted as clinically rather than minimally important differences
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