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This approach could be further improved by exploiting the well-known microneedle system, in which biodegradable polymers deliver fluid material by crossing biological barriers [296]

Engineering of gasdermin D-cleaving proteases Although the evolved X(GD_L) proteases displayed promising activity through profiling activity in the PACE architecture, they failed to yield measurable kinetic parameters or substrate cleavage in mammalian cells, likely due to insufficient catalytic activity (Fig