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The samples were vortexed 15 s and left in a freezer at -20 C for 60 min, followed by centrifugation at 21,100 RCF for 15 min at 4 C

Ongoing real-world monitoring and outcomes assessments help optimize treatment benefits and sustain market growth

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Free radicals in biology and medicine

Nanocarrier uptake in midbrain organoids Control or PD organoids were embedded in 10 l Geltrex (Thermo Fischer Scientific) at the bottom of 24-well plates (1 organoid/well), then culture inserts were placed above them containing the BBB model as described in our previous study [24]