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Materials and methods Establishment of PCOS mouse model Four-week-old female C57BL/6 mice (Guangdong Medical Laboratory Animal Center) were housed at 22 2C with a 12-h light/dark cycle and given standard feed and water ad libitum

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Binding/ Wash and Elution buffers are available, in addition to reduced glutathione for purification

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At high or chronic oxidative stress, however, the model suggests that the balance shifts towards GSSG, and removal of cysteine in the form of GSSG dominates, resulting in a decline in cysteine