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Quantitative reverse transcription polymerase chain reaction (qRT-PCR) The total RNA of the hypothalamus and hindbrain was extracted with TRIzol Reagent, while the RNA of brown adipose tissue and liver was extracted by RNeasy Plus Mini Kit (#74136
Adipose tissue is inherently pro-inflammatory, so losing weight naturally lowers inflammatory markers
Movement traces and quantification of locomotor activity (velocity and distance travelled) were obtained using Noldus EthoVision XT software (Noldus)
The vilon peptide research on immune and metabolic modulation provides complementary context for understanding how peptide-driven metabolic shifts operate across multiple body systems simultaneously
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