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The authors warn that a limitation of SOUL is that the trial population was at higher cardiovascular risk than the general type 2 diabetes population, in whom around one third have established CVD and 2540% have CKD

Long-term metformin use is associated with B12 deficiency in approximately 10-30% of patients, with risk increasing with duration of therapy and higher doses

With CellTox Green Dye present, there was only a slight change in oxidized glutathione or total glutathione levels, indicating no adverse effect of multiplexing these two assays (compare green and gray bars, Figure 3, Panels B and C, Figure 4, Panel B and C)

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