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Separate-vials Reconstitution Reference Compound Cagrilintide Vial size 5 mg BAC water 2.0 mL Concentration 2.5 mg/mL Weekly draw at 2.4 mg 0.96 mL = 96 units U-100 Compound Cagrilintide Vial size 10 mg BAC water 3.0 mL Concentration 3.33 mg/mL Weekly draw at 2.4 mg 0.72 mL = 72 units U-100 Compound Semaglutide Vial size 5 mg BAC water 2.0 mL Concentration 2.5 mg/mL Weekly draw at 2.4 mg 0.96 mL = 96 units U-100 Compound Semaglutide Vial size 10 mg BAC water 3.0 mL Concentration 3.33 mg/mL Weekly draw at 2.4 mg 0.72 mL = 72 units U-100 Volumes near 1 mL exceed standard U-100 / 0.3 mL syringe capacity

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The following day, assay media was prepared by supplementing specialized DMEM pH 7.4 (Agilent, 103575-100) with 10% dFBS, 1% penicillinstreptomycin, 25 mM glucose and 2 mM L-glutamine

BPC 157 treatment resulted in the up-regulation of Egr1, Akt1, Kras, Src, Foxo, Srf, Vegfr2, Nos3, and Nos1 and the down-regulation of Nos2 and Nfkb (Mapk1 was not activated)