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coli LOBSTR [Andersen et al.,2013] cells carrying a pETDuet-1 vector encoding codon optimized, C-terminally His-tagged RagA with a mutation (T21N) that favors the GDP-loaded state [Yang et al.,2020,Kim et al.,2008,Shen et al.,2017] and tagless wildtype (WT) RagC in its state (Addgene: 99664), were grown in Terrific broth (TB) and protein expression induced with an overnight IPTG treatment at 18 C b

According to the post-hoc analysis of SURPASS-3 (NCT03882970), 52 weeks of tirzepatide treatment resulted in a 4.4% reduction in MFI in individuals with T2DM [85]

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