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The copper uglies occur when chronic GHK-Cu overuse upregulates matrix metalloproteinases (MMPs) past the healthy remodeling threshold
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Then, 20 L of MTT solutions was added to each well followed by an incubation period of 3 hours, then the media were removed and 200 l of DMSO was added to dissolve the crystal and the absorbance was recorded at 570 nm (BioTek, USA) and cell viability was calculated as a percentage compared to cells treated with DMEM alone
The two peptides address tissue repair through complementary mechanisms, and combining them produces faster and more complete healing than either produces alone
Kannan R, Mittur A, Bao Y, Tsuruo T, Kaplowitz N
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