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Sulfate, free and esterified were measured by a modification of the nephelometric method of Berglund and Sorbo [38] and urine creatinine using a kit (555-A) from Sigma

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Notably, RT-qPCR analysis showed a sharp increase in OCT4 mRNA expression, a marker of pluripotent stem cells and essential for TIC self-renewal [33], in the colonies formed in 3D gels relative to 2D culture conditions (Fig