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To this end, BALB/c mice, which are genetically deficient in endogenous melatonin production (28), were intranasally challenged with 25 g Alternaria alternata (AA) or PBS daily for four days, with or without concurrent intraperitoneal melatonin treatment using established protocols described previously (2931) ( Figure 1A )

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The construct containing the SP of effector Avr1b were used as positive controls as previously described 79

These imperfections can linger long after the blemish itself has healed