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doi: 10.1016/j.neuron.2024.10.003 49 ShiYXuNLiuBMaYFuXShangYet al

To increase selection stringency, the RBS strength in front of gIII -neg was gradually enhanced over the subsequent PANCE passages (Extended Data Fig

Unlike plain sterile water, which must be used immediately after opening due to contamination risk, bacteriostatic water is designed for multi-dose use, the benzyl alcohol inhibits microbial growth, allowing the vial to be accessed multiple times over a 28-day period while maintaining sterility