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Always verify your syringe type matches your math

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It should be noted that GSH data obtained from post-mortem brain samples are variable in quality, as brain GSH is affected by many pre- and post-mortem factors and changes quickly after death [19, 20]

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The reaction was carried out for 15 min in 2 ml microcentrifuge tubes at 30 C, 1,200 rpm using a 3 mm shaking diameter