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Adipose tissue biopsies and SA--gal activity During bariatrics surgery, paired visceral adipose tissue was harvested and using the Senescence Cells Histochemical Staining Kit (Sigma, St Louis), fresh adipose tissue samples were examined for SA--gal activity ( 3 The ratio of cyan pixel intensity to the total number of pixels in the biopsy region, multiplied by 1,000, was used to calculate the SA-gal values, which were then reported as arbitrary units (AU)

There are two main ways this is done, centrifugation and membrane filtration, and they lead to very different outcomes

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