In db/db mice, activation of Nrf2 alleviated TECs ferroptosis, tubule damage and kidney injury (195)
Briefly, sodium bicarbonate, glucose, sodium pyruvate, and all 20 amino acids (except cystine and serine), biotin (B4639, Sigma), and vitamin B12 (V6629, Sigma) were added to the DMEM medium (#D9800-28, USBiological, Salem, MA) to match standard IMDM, supplement with 15% human AB serum and 15% dialyzed FBS (#SH300790340, Cytiva Hyclone), along with 10 ng/mL interleukin-3, 50 ng/mL stem cell factor and 2IU/mL erythropoietin
& Jan, Y
Studies have shown a direct correlation between glutathione levels and cancer proliferation and metastatic activity
Open-Field Test The open-field test measured exploratory behavior and anxiety levels