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Cells produce ROS during normal aerobic respiration, and superoxide dismutase (SOD) in the cells catalyzes the conversion of superoxide anions (O 2 ) into hydrogen peroxide (H 2 O 2 ) and oxygen (O 2 ), thereby detoxifying ROS
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Therefore, the GSSG is detected by measuring the absorption (max = 412 nm) of DTNB (5,5 Edithiobis (2-nitrobenzoic acid) ) using the enzymatic recycling system
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