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Acta Otolaryngol
doi: 10.3389/fphar.2022.1055296 269
Briefly, 2030 oocytes were lysed on ice in mammalian lysis buffer 5X (ab179835, Abcam), then deproteinized, neutralized, and diluted to concentrations suitable for standard curve fitting
This size is recommended so that the needle reaches the muscle tissue where the B12 can be absorbed effectively
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