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75 g of protein lysates were heated for 10 min at 95 C in Laemmli 6X SDS sample buffer (Boston BioProducts, # BP-111R) with 5% 2-mercaptoethanol (VWR Life Science #M131-100ml) and then ran on 420% Criterion TGX pre-cast gels (Bio-Rad #5671093)
It is recommended to store them in a cool, dry environment, typically refrigeration or freezing, to preserve their efficacy
The peptides for perimenopause guide and peptides for women over 40 provide additional context for female-specific protocols
MOTS-c is a short peptide encoded within mitochondrial DNA
The assay diluent was then added to the plates and incubated for 1 h on a shaker to block nonspecific binding
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