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The supernatant was discarded while the sediment nanoparticles were washed firstly with water to remove excess PVA and then with ethanol to remove excess dodecylamine
It contributes to that fuller, more pumped look your muscles get, and it may even play a role in muscle growth signaling
Every 5th fraction was combined into a new Eppendorf to make five final fractions that were dried using a SpeedVac vacuum concentrator
Timeline of typical observations Week 1-2: Minimal visible changes
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