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The shift of IR absorption peaks in the SEIRA spectra is due to interaction of the target molecule with metal nanoparticles, especially related to the orientation of target molecule on the metal surface ( 1 ) is missing in the surface-enhanced IR absorption spectrum of glutathione, which is the reaction of the silver nanoparticles with the sulfhydryl group in glutathione to form AgS bonds

Hanna MG, Miller JAL, Rooks D, et al

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However, the extent of ROS generation significantly differed that posttreatment inhibited the ROS generation by 58.4%, whereas pretreatment suppressed the generation of ROS by 90.5%