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The membrane was then incubated at room temperature in blocking solution for 1 hour, followed by incubation in blocking solution containing the appropriate dilution of either phospho-Jak2 or the primary antibody for growth hormone receptor, for 2 hours
Furthermore, while enzymatic removal of the L585 residue decreased FcRn binding for fusion designs with WT albumin, this was not the case for the corresponding QMP-engineered variants
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