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The following agents were used as positive controls: cytomix (known inflammatory-inducing agent, prepared using 500 ng/mL TNF-, 0.2 mg/mL lipopolysaccharide, 1% FCS, tissue exposure via liquid basal), Triton X-100 (cytotoxic at 10%, via liquid basal exposure) or IL-13 (a goblet cell hyper-, metaplasia agent at 10 ng/mL, via liquid basal exposure)
Magorzata Szczuko, Pomeranian Medical University, Poland Updates Copyright 2022 Wang, Li, Mao, He, Zhu and Wei
Use within the timeframe appropriate for your solvent choice, typically 2 to 4 weeks for bacteriostatic water or longer for acetic acid
for sensitive skin, start with once daily use and perform a patch test on a small skin area
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