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Combined effect is widely described as synergistic in preclinical and pharmacology literature
Cell viability evaluated by MTT assay Viability of the cells was assayed using cell proliferation Kit I with the absorbance of 595 nm being detected using a Perkin-Elmer Victor plate reader
Autophagy 12 (8), 14251428
At the end of treatment, mice were euthanized by asphyxiation with CO 2 and peripheral blood, spleen single cell population were analyzed for the ROS, ferroptosis levels by DCF and Bodipy-C11 staining followed by flow cytometry analysis
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