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Hence, a peptide was designed by substituting the negatively charged residues D42, D48, D49, E51, E56, and D57 within the p53 TAD PEP with alanine to increase its binding affinity to FOXO4 FHD and enhance membrane penetration
About 8 h after transfection, thymidine (100 mM aqueous stock solution, final concentration 2 mM) was added to the cells and incubated for 16 h
For musculoskeletal, tendon, ligament, or systemic targets, injection is the better-supported route because all relevant animal healing studies used injected BPC-157, and systemic absorption from capsules in humans is unverified
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