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After three washes with PBS, the cells were incubated with an Oil red O solution (Sigma-Aldrich) for 10 min

NMDA receptors are associated with glutamate, an essential excitatory neurotransmitter in the brain, while GABA receptors are linked to inhibitory neurotransmission, which is considered crucial for reducing neural activity and inducing a relaxed state

SFN protects against diabetic cardiomyopathy through increased NRF2 expression and correction of oxidative stress-induced modulation of the LKB1/AMPK pathway (259)

doi:10.1016/j.ab.2003.07.023

Its main function is to neutralise free radicals and reduce oxidative stress, protecting cells from damage caused by environmental factors