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severe thiamine deficiency, while rare, can cause Wernickes encephalopathy in hyperemesis gravidarum, and inadequate B is associated with anemia and neurologic issues in the newborn

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Scratch-wound assays, capillary tube-formation assays, and other in vitro endothelial preparations have all been used to characterise combination effects, with published research documenting outcomes that suggest additive or synergistic activity in many model systems

For reconstitution protocol, see How to Reconstitute Peptides