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Highlights: Purified from Schistosoma japonicum Useful as a molecular weight marker in gel analysis of GST-tagged proteins Suitable as a standard or control in ELISA and pull-down assays involving GST-tagged proteins Ideal for testing tag-specific cross-reactivity of antibodies in ELISA Specifications Specifications Safety and Handling For Research Use Only
In contrast, an increase in sub-visible particle count was seen after 21 days, and an increase in ThT fluorescence was seen after 28 days, in the case of co-formulation 1 containing HP-B-CD (Average MS: 0.92)
La duracin depende de la condicin siendo abordada y cmo usted responde
The proteins were eluted with 0.2 mg/ml FLAG-peptide in 20 mM HEPES pH 7.5, 100 mM NaCl buffer, then mixed with Laemmli sample buffer (BioRad), subjected to SDS-PAGE and Western blotting and detected by monoclonal ANTI-FLAG M2-peroxidase (HRP) antibody (1:2000) (A8592, Sigma-Aldrich, RRID: AB_439702) for SH3-FLAG and polyclonal A1CT antibody 58 (1:5000) and HRP-conjugated ECL Rabbit IgG (1:5000) (NA9340, Amersham, RRID:AB_772191) for arr1