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Tissues subjected to repeated freeze-thaw cycles were homogenized at 20 L/mg tissue of cold water in a 2 ml Eppendorf tube using a metal bead beater operating at 30 Hz for 5 min in a TissueLyser III (Qiagen)
We used the Frisn staging scale to rate papilledema severity, categorizing the swelling of optic discs from stage 0 (no papilledema) to stage 5 (severe papilledema) [19]
P.KatabiN.MitchellJ
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