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A pRS315- HS-pGSH1-URA3-GFP-tADH1-HS plasmid was subsequently constructed using these and GFP (above) fragments, with the same Gibson approach as described earlier
However, the relationship between TFR1 expression in the glomerular mesangial region and iron dysmetabolism in mesangial cells is still not fully understood, and the reason for the close association of TFR1 with ferroptosis in IgAN patients still needs to be explored
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