Statistics and reproducibility Statistical significance was determined using independent t -test for immunohistochemical and western blot analyses, and one-way analysis of variance for cell cycle and apoptosis detection by GraphPad Prism 7.0 software (GraphPad, Inc., La Jolla, CA, USA)
We identified 48 protein spots in 2-D gel analysis and selected proteins were divided into 64% collagen components and 30% non-collagen components as shown by the MALDI-TOF analysis
Some Risk: Confirmed use of alcohol during pregnancy with use less than High Risk or unknown usage patterns
Layered clothing is the most effective immediate intervention, allowing adjustment to varying environmental temperatures throughout the day
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