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Quantitative RT-PCR was carried out using a Step One Plus Real-Time PCR system (Applied Biosystems) with the SsoAdvanced Universal SYBR Green PCR Core Reagents Supermix (Biorad)
Although numerous peptides participate in this axis, glucagon-like peptide-1 (GLP-1) plays a central role
By looking at the "bigger picture," you move away from chasing isolated markers and toward a more informed, productive conversation with your healthcare team
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