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Anti-inflammatory and Antioxidant Effect of Poly-gallic Acid (PGAL) in an In Vitro Model of Synovitis Induced by Monosodium Urate Crystals
Because peptide therapy causes none of the side effects associated with HGH
The concentration of TNF- and IL-6 in the supernatant and serum harvested above was measured by using enzyme-linked immunosorbent assay kits (Dakewe, Beijing, China) according to the manufacturer's instructions
Cell Culture HepG2 cells were cultured in collagen-coated plates and maintained in DMEM containing 10% FBS, 1% penicillin/streptomycin (PAA Laboratories, Austria), 1% ultraglutamine (Lonza, Switzerland) and 1% non-essential amino acids (Sigma-Aldrich, Germany)
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