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At the base of the bulb is the papilla, which contains blood vessels

Moreover, in contrast to tap or distilled water, there are no chemicals, salts, ions, preservatives, or additives in SWFI

Euthanasia of animals was performed after the tests by immersion in cold water (0 to 4 C) until the cessation of the movements, to ensure death by hypoxia according to the AVMA Guidelines for the Euthanasia of Animals (Leary et al., 2020)

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Additionally, the N-terminal tryptophan is among the more oxidation-prone and light-sensitive residues