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The hCTR cells were cultured in growth medium consisting of Dulbeccos Modified Eagles Medium (DMEM, Gibco, #31966-021) supplemented with 10% v/v foetal bovine serum (FBS, Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.6 mg/mL Geneticin G418 (Gibco, #10131-027) and 0.2 mg/mL Hygromycin (Invitrogen, #10687010)

2.1.2 AP AP, 4,5,7-trihydroxyflavone, is a flavonoid mainly derived from parsley and chamomile that has been used as an antioxidant and antiinflammatory agent for many years, and recorded as a traditional Chinese medicine to interfere with the various disease progression such as cancer (Madunic et al., 2018

Most clinics add vitamin B12 (cyanocobalamin or methylcobalamin) to the blend and administer the whole thing as a single intramuscular or subcutaneous injection, which is why MIC injections and MIC B12 injections usually refer to the same product

W.YuanY.FuY.WangP.et al (2021)

It is used for the detoxification of electrophilic metabolites