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We hypothesize that the excess GSSG in cytosol were transported to extracellular space where class III peroxidases locate and served as the oxidant either directly or indirectly during ROS production by class III peroxidases (Figure 6)

Implementation: Dont go below 1200 kcal for women or 1500 kcal for men (adjust for body size) Better approach: calculate maintenance calories, subtract 500-750 kcal for deficit Track intake for the first few weeks (appetite suppression can mask dangerous undereating) Strategy 6: Micronutrient Support B vitamins are cofactors in ATP production and nerve function

Data are normalized by CS protein levels

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It also increased levels of Faecalibacterium prausnitzii, a key butyrate-producing bacterium with anti-inflammatory properties