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Abd El Aziz MS, Kahle M, Meier JJ, Nauck MA
IGF-1 LR3 is modified at two positions (N-terminal 13-amino-acid extension and Arg3 substitution) to substantially reduce IGFBP-3 binding, extending preclinical activity duration well beyond the roughly 10 to 12 minutes seen with unbound native IGF-1
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After that, cells expressing GIPR and GLP-1R were incubated with 1:300 anti-Flag primary antibody (diluted with PBS containing 5% BSA, Sigma), and those expressing GCGR were incubated with 1:50 anti-GCGR antibody (diluted with PBS containing 5% BSA, Abcam) at RT for 1 h
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