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Separation was achieved using a gradient of 10 mM ammonium acetate in water (mobile phase A
The problem, though, is that it's very unstable, turns brown and becomes ineffective in no time (after a few month) and the cosmetics industry is trying to come up with smart derivatives that are stable and have the magic properties of pure Vitamin C
PMC12313605 Staresinic, M., et al
If you find that Vitamin C on an empty stomach causes discomfort, try a buffered version or take it with a very light snack
With B12 injections, you also have to be careful not to inject them into a vein
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